Reproductive Toxicology

Effects of sublethal ingestion of toxic substances typically do not result in mortality, as evaluated in the short-term LD50 and LC50 studies. Long-term, sublethal exposure may result in reduced reproductive potential characterized by reduced egg production, reduced fertility, decreased embryo viability, low hatch rates or low fledging success. Long-term effects on the parental colony are also evaluated.

Avian Testing Component

To evaluate potential reproductive effects of products under review for registration, Springborn conducts regulatory testing under FIFRA Avian Reproductive Test (Series 71-4), OPPTS Avian Reproduction Test 850.2300 (http://www.epa.gov/oppts/pubs/frs/home/guidelin.htm), and OECD Avian Reproduction Test 206 (http://www.oecd.org/document/40/0,3343,en_2649_34377_37051368_1_1_1_1,00.html). A standard reproduction test designed to meet these regulatory guidelines requires a minimum of 30 weeks in-life.

Standard species required for reproductive toxicity testing are the mallard and/or northern bobwhite. Because reproductive toxicity tests are long, cost- and resource-intensive studies, Springborn frequently performs preliminary range-finding studies prior to definitive testing. By defining the most appropriate test concentration range for the larger, long-term study, our client's investment is better positioned to return the most useful toxicity data for their registration requirements and incorporation into risk assessment models.

Analytical Verification Component

Reproductive toxicity studies incorporate analytical chemistry and residue analysis of the test diets. Springborn maintains two separate analytical facilities that can accommodate the dietary analyses requirements of the avian dietary studies. Springborn is able to either develop analytical methodology tailored to the project's specifications, or follow methodology provided by the client.